Submitted to: American Phytopathology Society
Publication Type: Abstract Only
Publication Acceptance Date: March 9, 1999
Publication Date: N/A
Technical Abstract: A new maize-infecting virus, isolated from Arizona-grown plants, incited chlorotic spots, streaks and spindle-shaped rings with diffuse, necrotic margins on Seneca Chief sweet corn leaves following vascular puncture inoculation of kernels. The virus was not transmitted by rub inoculation, aphids nor selected leafhopper species. Purified virions were isometric (28 nm diam.) and contained a 31 kDa putative capsid protein and single-stranded RNA. High concentrations of the largest RNA (ca. 4.3 kb) were observed in preparations of leaf RNA from symptomatic plants. Randomly primed cDNA clones of viral RNA hybridized with RNAs from virus-infected but not from healthy, leaves. Sequence comparisons indicated the cDNAs had significant homology with the replicase and capsid genes of several tombusviruses. The data indicated the identification of a new maize-infecting virus, named maize necrotic streak virus, tentatively belonging to the Tombusviridae.