|Sonstegard, Tad - FORMERLY 5438-01-30|
Submitted to: Journal of Animal Science Supplement
Publication Type: Abstract Only
Publication Acceptance Date: January 14, 2000
Publication Date: December 20, 2000
Citation: Vallet, J.L., Smith, T.P., Sonstegard, T.S., Heaton, M.P., Fahrenkrug, S.C. 2000. Characterization of the gene for porcine endometrial/placental membrane folate binding protein (mFBP) [abstract]. Journal of Animal Science. 78(Supplement 2):77. (Abstract No. 231) Technical Abstract: The developing conceptus and the fetal erythron require high levels of folate. Endometrial cDNAs for secreted and membrane folate binding proteins have been characterized. Half the mFBP gene was subcloned from a yeast artificial chromosome. The rest of the gene (bases 5607 to 9106) was obtained from a bacterial artificial chromosome clone. The mFBP gene is composed of 5 exons (exon 1 = 68 bp, bases 1797-1864; exon 2 = 194 bp, bases 5312-5505; exon 3 = 189 bp, bases 6879-7067; exon 4 = 136 bp, bases 8070-8205 and exon 5 = 421 bp, bases 8350-8770). The previously reported heterogeneity in the 5' end of the mFBP mRNA corresponded to transcription starting at exon 1 or 2, suggesting that the mFBP gene has two promoters. Possible transcription factor (TF) binding sites 5' of exon 1 included: CCAAT binding factor (CBF) sites at 1016, 1352, and 1542 bp, c-myb sites at 259 and 1069 bp, myc associated zinc finger protein (MAZ) sites at 644 and 988 bp, and a peripherin-2 site at 1669 bp. Possible TF binding sites 5' of exon 2 included: CBF sites at 2015, 4112, and 4847 bp, an activator protein (AP)-1 site at 2269 bp, AP-2 sites at 3070, 4011, 4371, and 5160 bp, a c-myb site at 5285 bp, a c-myc site at 2685 bp, lymphocyte factor-1 sites at 3296 and 4827 bp, and MAZ sites at 2678, 2888, 3078, and 3365 bp. These results suggest candidate TF controlling the mFBP gene. Manipulation of these factors may improve delivery of folate during pregnancy, resulting in increased litter size in swine.