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United States Department of Agriculture

Agricultural Research Service

Title: Separation and Quantification of Two Fluoroquinolones in Serum by on-Line High-Performance Immunoaffinity Chromatography

Authors
item Holtzapple, Carol
item Pishko, Elizabeth
item Stanker, Larry

Submitted to: Analytical Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 22, 2000
Publication Date: September 1, 2001

Interpretive Summary: Fluoroquinolones are drugs that are used by the livestock industry to prevent disease due to bacterial infection. Although these drugs are useful for keeping animals healthy, drug residues should not be present in animal products that are brought to market. In order to detect improper use of these important drugs, a simple, automated test was developed to detect residues of these drugs in cattle serum. This test is an improvement over previously reported rapid tests because use of environmentally unfriendly chemical solvents are eliminated. Rapid tests such as the one described here should help producers, as well as government agencies, to screen cattle for the presence of fluoroquinolone residues.

Technical Abstract: To demonstrate that the two structurally similar chemicals can be extracted from a complex matrix and then separated from each other on the basis of their relative affinities for an antibody, an automated column switching system was used incorporating on-line, high-performance immunoaffinity chromatography (HPIAC). By using an on-line cleanup column in tandem with an HPIAC column, the fluoroquinolones could be separated from the serum matrix and then separated from each other based upon their affinity for Mab Sara-95 without the use of organic solvents or reversed- phase liquid chromotography (RPLC). This method demonstrates true immunoaffinity separation of structurally related compounds in a complex matrix.

Last Modified: 10/24/2014