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Submitted to: Book Chapter
Publication Type: Book / Chapter Publication Acceptance Date: 8/5/1999 Publication Date: N/A Citation: Handler, A.M. 2000. An introduction to the history and methodology if insect gene transfer. Book Chapter. pps. 3-26. Interpretive Summary: The ability to achieve gene transfer in economically important insects is a major goal of our laboratory at the CMAVE. Development of this methodology and strategies to effectively and safely utilize transgenic insects for biological control has and will depend upon collaborative interactions and communication among a wide variety of scientists. This review article will serve to educate many of the researchers who may wish to use this technology, but presently have a limited background regarding the history and methodology of insect gene transfer. It discusses early attempts at gene transfer before molecular tools were available, and more recent attempts and successes using mobile genetic elements as vectors and the variety of genes used for transgenic selection. A detailed review is made of specific techniques currently used for insect trasnformation. Technical Abstract: The ability to achieve gene transfer in economically important insects is a major goal of our laboratory at the CMAVE. Development of this methodology and strategies to effectively and safely utilize transgenic insects for biological control has and will depend upon collaborative interactions and communication among a wide variety of scientists. This review article will serve to educate many of the researchers who may wish to use this technology, but presently have a limited background regarding the history and methodology of insect gene transfer. It discusses early attempts at gene transfer before molecular tools were available, and more recent attempts and successes using mobile genetic elements as vectors and the variety of genes used for transgenic selection. A detailed review is made of specific techniques currently used for insect trasnformation. |
