Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: October 30, 2000
Publication Date: January 9, 2000
Citation: Pan, Y., Burner, D.M., Legendre, B.L. 2000. Search for dna markers to assist sugarcane breeding [abstract]. Plant and Animal Genome VIII Abstract. W120:29. Technical Abstract: Sugarcane can self-pollinate during cross attempts. It is often difficult to distinguish selfs and hybrids due to the lack of reliable morphological markers and limited genetic knowledge. Studies are being conducted to develop species-specific PCR and RAPD markers to assist in the selection of interspecific sugarcane hybrids. Although the nucleotide sequences of 5S rRNA intergenic transcribed spacers were not sufficiently divergent among sugarcane cultivars, Saccharum officinarum, and S. spontaneum, there was sufficient nucleotide sequence variation in Erianthus spp. and S. giganteum to allow for the design of specific PCR primers. Primers Eri3/Eri4 primed only the amplification of a 550 bp PCR product from Erianthus spp. clones; primers Gig2/PI primed the amplifications of a 176 bp PCR product from all known cytotypes of S. giganteum (2n = 30, 60, and 90) and also a 400 bp PCR product from Erianthus spp. clones. Several species-specific RAPD markers were identified. These included OPA-10g (185 bp) for S. brevibarbe var. contortum, OPA-10d (465 bp) for S. giganteum cytotype 2n = 30, and OPA-11-366 for sugarcane cultivars LCP 85-384 and CP 62-258. One RAPD marker, OPA-11-366, was successfully used to identify hybrids in two progeny populations derived from crosses of S. spontaneum clone Djatiroto with sugarcane cultivars LCP 85-384 and CP 62-258. Results from a recent study on nucleotide sequence variability among various intergenic transcribed spacers of the 18S-26S rRNA locus in sugarcane and its closely related taxa will be discussed.