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United States Department of Agriculture

Agricultural Research Service

Title: Combinatorial Approaches to Optimizing Critical Fluid Processing

Authors
item King, Jerry
item Taylor, Scott
item Eller, Fred
item Snyder, Janet

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: September 28, 1999
Publication Date: N/A

Technical Abstract: Over the past two decades, critical fluid technology has evolved to embrace many technical disciplines and finds application not only in the extraction mode but in fractionation and reaction modes. In this presentation, we couple methodologies to rapidly optimize conditions for critical fluid extraction, reaction, and fractionation. Sequential or parallel processing can be carried out on both commercial and home-built instruments. Analytical instrumentation designed for supercritical fluid extraction (SFE) allows the rapid alteration of experimental conditions and avoids the use of excess fluid and reagents. The process can be scaled up to approximately 100-150 grams of substrate. Extraction of cedarwood has been studied using a sequential SFE processor. The effect of extraction pressure, temperature, time, and substrate preparation were determined utilizing the microprocessor controller on the extractor which permitted running up to 24 samples/day. Similarly, phospholipids were enriched from lecithin using various sorbents for preparative supercritical fluid chromatography (SFC). Optimized parameters for enrichment included using multiple cartridges packed with different sorbents under different conditions. Analytical SFE instruments have also been employed for the inverse screening of catalysts, such as lipases for the esterification of lipid substrates. A sequential approach to studying the action pattern of various lipases under supercritical carbon dioxide was utilized. These examples illustrate the possibilities for combinatorial assessment of processing conducted in the presence of critical fluid media.

Last Modified: 8/22/2014
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