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United States Department of Agriculture

Agricultural Research Service

Title: Use of Pkh2 to Demonstrate Antigen-Specific B Cell Unresponsiveness in Cattle Chronically Infected with Mycobacterium Avium Subsp. Paratuberculosis

item Pesch, Bruce
item Stabel, Judith
item Waters, W - IOWA STATE UNIV., AMES
item Sacco, Randy
item Wannemuehler, M - IOWA STATE UNIV., AMES
item Harp, James

Submitted to: Great Lakes International Imaging and Flow Cytometry Association
Publication Type: Abstract Only
Publication Acceptance Date: October 2, 1998
Publication Date: N/A

Technical Abstract: Mycobacterium avium subsp. paratuberculosis infection of cattle results in a chronic granulomatous enteritis. Clinical disease is preceded by a lengthy subclinical infection. In this study, simultaneous PKH2 and surface phenotypic labeled peripheral blood lymphocytes were used to compare responses of control, subclinically, and clinically infected animals. B cells from animals with subclinical paratuberculosis responded well to stimulation with M. avium subsp. paratuberculosis. B cells from controls and clinically infected anmals did not respond to antigen stimulation although responses to Concanavalin A mitogen stimulation were normal. Animals with clinical disease also had higher percentages of B cells than either controls or subclinically infected animals.

Last Modified: 4/19/2015