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United States Department of Agriculture

Agricultural Research Service

Title: Restriction Enzyme Analysis of Bordetella Bronchispetica Isolates

Authors
item Sacco, Randy
item Register, Karen
item Nordholm, Gwen

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: October 7, 1999
Publication Date: N/A

Technical Abstract: Bordetella bronchiseptica is a common respiratory pathogen in a number of animal species. It is an etiologic agent of swine atrophic rhinitis and bronchopneumonia, canine tracheobronchitis, and bronchopneumonia in laboratory and companion animals. Previously, it was shown that the technique of ribotyping could be utilized to characterize isolates of B. bronchiseptica. Those studies documented the existence of 19 unique Pvu I ribotypes from more than 200 isolates examined. In the present study, we examined the utility of restriction enzyme analysis (REA) to further characterize B. bronchiseptica isolates previously typed on the basis of ribotype profile. In the present study, a total of 195 B. bronchiseptica isolates from 12 different host species worldwide were examined by restriction enzyme analysis. We found digestion of chromosomal DNA from these isolates with Hinf I, followed by gel electrophoresis and ethidium bromide staining, produced 48 distinct fingerprint profiles. REA of these isolates using Alu I resulted in 38 fingerprint profiles. While REA frequently provided more discriminatory power than ribotyping, there were examples where the use of ribotyping was more discriminatory than REA. Based on these results, the combination of REA and ribotyping should prove useful in epidemiological studies of B. bronchiseptica isolates.

Last Modified: 10/25/2014
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