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United States Department of Agriculture

Agricultural Research Service

Title: Using 16s Rdna Sequences to Identify Xylella Fastidiosa

Authors
item Banks, D - FL A&M UNIV
item Jarret, Robert
item Newman, M
item Chang, C - UNIV OF GA
item Smith, Barbara

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: June 1, 1999
Publication Date: N/A

Technical Abstract: The nucleotide sequences of the small sub-unit ribosomal RNA genes (16S rDNA) were used to identify Xylella fastidiosa, a nutritionally fastidious bacterium. The 16S rDNAs from nine strains including seven pathotypes of Xyl.fastidiosa and one strain of Xanthomonas campestris pv. campestris were amplified through PCR using two conserved primers (forward primer 5'-AGA GTT TGA TCA TGG CTC AG-3' and reverse primer 5'-AAG, GAG GTG TGA TCC AGC CG-3') and sequenced. Partial sequences (200 bp from 5' end) were compared with entries in the GenBank DNA database, including a Xyl.fastifiosa 16S rDNA sequence (M26601). All Xyl. fastidiosa sequences identified M26601 as the most similar sequence (199/200 or 99.5%). The Xanthomonas rDNA sequence identified a Xanthomonas 16S rDNA as the most similar sequence, contrasting to M26601 as the 50th (106/109 or 97.2%). The fact that Xyl. fastidiosa strains are highly homologous in their rDNA sequences indicates that 16S rDNA sequences can be used as a signature character to identify this bacterium.

Last Modified: 4/19/2014
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