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United States Department of Agriculture

Agricultural Research Service

Title: Cytological and Fluorescent in Situ Hybridization (Fish) Sutdies on Anther Culture-Derived Haploid and Aneuploids of Durum Wheat

item Altuntepe, Munevver - CUKUROVA UNIV, TURKEY
item Peterson, Terrance
item Jauhar, Prem

Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: September 1, 1999
Publication Date: October 15, 1999
Citation: Altuntepe, M., Peterson, T.S., Jauhar, P.P. 1999. Cytological and fluorescent in situ hybridization (fish) sutdies on anther culture-derived haploid and aneuploids of durum wheat. Agronomy Abstracts, p. 83.

Technical Abstract: In an attempt to produce haploids of durum wheat, we produced several plantlets in Turkish cultivars by anther culture. Somatic and meiotic chromosomes were studied, using both conventional and fluorescent staining techniques. The haploids with 14 chromosomes were all albinos. Some albino regenerants had 28 chromosomes. Green plantlets generated from the same callus (probably originating from a single microspore) had 28 to 70 somatic chromosomes. These plants showed several abnor-malities, which increased with the level of polyploidy. An interesting feature was the preferential multiplication of B-genome chromosomes, which formed bivalents and multivalents (trivalents, quadrivalents and hexavalents). Both intragenomic and intergenomic multivalents were observed. Translocations involving the A- and B-genome chromosomes were also recorded. Chromosome breakages resulting in fragments and telocentrics, and dicentric chromosomes were observed. Chromosome multiplication and other aberrations induced during culture may form the bases of somaclonal and gametoclonal variations.

Last Modified: 4/22/2015
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