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ARS Home » Research » Publications at this Location » Publication #104406
Title: FORMATION OF INFECTION CUSHIONS BY SCLEROTINIA MINOR ON A CELLOPHANE MEMBRANE IN RESPONSE TO PEANUT STEMS FROM RESISTANT AND SUSCEPTIBLE CULTIVARS

Author
item Melouk, Hassan
item ABOSHOSHA, S - COL. AGRIC., ALEXANDRIA

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 8/7/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Four peanut shoots (12 cm), from 8-wk-old plants, were placed in a water moistened cellophane bag, which was placed upright in a styrofoam cup (177 ml ca.) filled with 15 g perlite. One hundred ml of fragmented S. minor- mycelial suspension (1 g mycelia; fresh wt.) was added to each cup. Cups were placed in a humidity chamber at 100% RH for 5 days at 27 deg C. Cellophane bags were then carefully lifted, and outer surface of the cellophane facing the perlite was gently rinsed with cold running water for 2 min. Ten 1-cm**2 sections of the cellophane were placed on slides with the inner surface of the cellophane contacting the glass. Squares were stained with lactophenol cotton blue for 3 min, mounted in a glycerin gel, and infection cushions (IC) counted. IC/cm**2 of cellophane was 3.8 in response to Okrun, a sclerotinia-susceptible peanut cultivar, whereas the IC in response to Southwest Runner, a sclerotinia-resistant peanut, was 2.7 7with an LSD(0.05) of 0.5.