Submitted to: Entomological Society of America Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: November 15, 1999
Publication Date: N/A
Technical Abstract: The braconid parasitoid wasp Glyptapanteles indiensis infects its natural host, gypsy moth (Lymantria dispar), with a polydnavirus (GiPDV) to suppress the immune system during parasitization. DNA from the GiPDV genome has been shown to transform cell lines derived from gypsy moth in vitro and is also capable of transforming in vitro to varying degrees lepidopteran [cabbage looper (Trichoplusia ni), fall armyworm (Spodoptera frugiperda), Indian meal moth (Plodia interpunctella), tobacco budworm (Heliothis virescens)] and coleopteran, corn rootworm (Diabrotica undecimpunctata), insect cell lines derived from various somatic tissue types. The unusual ability of GiPDV DNA to persist within and transform insect cell lines is of interest to us. We have determined that partial GiPDV DNA is maintained in transformed gypsy moth cells in an integrated state. Here, we isolate and compare site(s) for GiPDV DNA integration within the chromosome of insect cell lines derived from the natural host and examine molecular characteristics of viral persistence in a non-host lepidopteran cell line.