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United States Department of Agriculture

Agricultural Research Service

Title: An Improved Procedure for Extracting DNA for Use with a Microsatellite Marker Associated with the Waxy Gene

Authors
item Bergman, Christine
item Delgado, J - TEXAS A&M UNIV
item Fjellstrom, Robert
item McClung, Anna
item McClung, Anna

Submitted to: Experiment Station Bulletins
Publication Type: Experiment Station
Publication Acceptance Date: May 1, 1999
Publication Date: N/A

Technical Abstract: A microsatellite marker has been reported that strongly correlates with the various classes of apparent amylose content in rice. This marker is a tool that has the potential to decrease the time required to develop new cultivars. However, adoption of this technology by the USDA Rice Quality Evaluation Program has been hindered do to the amount of time required to extract DNA and the toxic chemicals required for that process. Consequentl we have developed an improved procedure for extracting DNA from rice using standard laboratory equipment. The method uses sodium hydroxide, a less toxic chemical than that used in the previously reported procedure. It is also simpler and quicker. And because rice kernels (milled or brown) are used in the procedure, no different sample form or additional quantity of sample would need to be sent by breeders to the USDA Rice Quality Laboratory than what is currently sent. During the next phase of this research the new DNA extraction method in combination with the waxy microsatellite marker will be used to examine the relationship between the various classes of the waxy locus and the apparent amylose content of the 1999 Uniform Regional Rice Nursery entries and in a breeding population.

Last Modified: 8/22/2014