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United States Department of Agriculture

Agricultural Research Service

Title: Interlab Comparison of Simmondsin Analysis

Authors
item Abbott Dr, Thomas
item Holser, Ronald
item York, David - PENNINGTON BIOMEDICAL RES
item Flo, Gerda - KATOLIEKE UNIVERSITEIT

Submitted to: Industrial Crops and Products International Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: November 15, 1999
Publication Date: N/A

Technical Abstract: Eleven samples containing various amounts of simmondsin (S), simmondsin ferulate (SF), demethyl simmondsins (DMS), and didemethyl simmondsins (DDMS) were sent to four different laboratories for analysis. The samples were made from chromatographically pure simmondsin, animal feed formulations containing jojoba meal, defatted jojoba meal, water extracts of jojoba meal, and combinations of these ingredients. Where mixes were made, all materials were ground together in a coffee mill and sieved. Three labs analyzed for at least three components and one lab analyzed for only S. The means of the S, SF, DMS, and DDMS percentages in the samples were determined to be: sample #1, 96.0% S, 0 SF, 3.16% DMS, 1.23% DDMS; #2, 100% S, 0 SF, 0.36% DMS, 0 DDMS; #3, 31.9% S, 2.37% SF, 9.34% DMS, 4.92% DDMS; #4, 21.8% S, 1.78% SF, 7.46% DMS, 5.23% DDMS; #5, 0.72% S, 0 SF, 0 DMS, 0.02% DDMS; #6 7.22% S, 1.44% SF, 3.14% DMS, 2.14% DDMS; #7 16.9% S, 3.19%SF, 0 DMS, 0 DDMS; #8 12.9% S, 0.90% SF, 0.06% DMS, 0.04% DDMS; #9 4.73% S, 0 SF, 0 DMS, 0 DDMS; #10 44.9% S, 0 SF, 1.70% DMS, 1.13% DDMS; #11 8.17% S, 1.44% SF, 2.99% DMS, 2.09% DDMS. The implications of the amount of the components for these sources will be discussed. There were no significant differences between laboratories, but, with more analyses from other labs, smaller differences may appear statistically that do not appear with the results from these four labs.

Last Modified: 4/17/2014
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