ARS | Publication request: DEVELOPMENT AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES TO SUBGROUP J AVIAN LEUKOSIS VIRUS

Submitted to: American Veterinary Medical Association Abstract
Publication Type: Abstract Only
Publication Acceptance Date: July 12, 1999
Publication Date: N/A

Technical Abstract: A panel of monoclonal antibodies (Mabs) to subgroup J avian leukosis virus (ALV-J) was developed by fusion between NS1 and spleen cells from mice immunized with SF9 cells infected with recombinant baculovirus Hc1 env. Using indirect immunofluorescence assay (IFA), three of four Mabs reacted with ALV-J, but not with subgroups A, B, C, D, or E of ALV. Interestingly, ,one of the four Mabs reacted with exogenous, but not with endogenous ALV. Western blot and immunoprecipitation tests showed that molecular weight of ALV-J glycoprotein recognized by Mabs was about 90 kDa. These monoclonal antibodies can be useful in the diagnosis of ALV-J.