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United States Department of Agriculture

Agricultural Research Service

Title: Antigenic Characterization of Field Isolates of Subgroup J Avian Leukosis Virus

item Fadly, Aly
item Lee, Lucy
item Silva, Robert

Submitted to: American Veterinary Medical Association Abstract
Publication Type: Abstract Only
Publication Acceptance Date: July 12, 1999
Publication Date: N/A

Technical Abstract: Virus neutralization (VN), enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence (IFA) tests were used to study the antigenic variations among various field islolates of subgroup J avian leukosis virus (ALVJ). Results suggest that using the VN test, chicken polyclonal antibodies to field isolates failed to neutralize strain ADOL-Hc1 of ALV-J However, such antibodies tested positive using a commercial ELISA specific for detection of antibody to ALV-J. Using IFA, monoclonal antibody against strain ADOL-Hc1 of ALV-J reacted with most of the field isolates tested.

Last Modified: 4/21/2015
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