Submitted to: American Veterinary Medical Association Abstract
Publication Type: Abstract Only
Publication Acceptance Date: July 12, 1999
Publication Date: N/A
Technical Abstract: Virus neutralization (VN), enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence (IFA) tests were used to study the antigenic variations among various field islolates of subgroup J avian leukosis virus (ALVJ). Results suggest that using the VN test, chicken polyclonal antibodies to field isolates failed to neutralize strain ADOL-Hc1 of ALV-J However, such antibodies tested positive using a commercial ELISA specific for detection of antibody to ALV-J. Using IFA, monoclonal antibody against strain ADOL-Hc1 of ALV-J reacted with most of the field isolates tested.