Submitted to: Animal Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: August 28, 1999
Publication Date: N/A
Interpretive Summary: We are interested in the acid phosphatase 5 (ACP5) and the erythropoietin receptor (EPOR) genes because of their role in blood development in pig embryos. We have found these two genes to be close in pig genomic DNA by radioactive hybridization. We predicted EPOR to be on pig chromosome 2 because ACP5 is known to reside there. We confirm this prediction by hybridization of fluorescent EPOR probes to pig chromosome 2, in the same region ACP5 is found.
Technical Abstract: Our interest in acid phosphatase 5 (ACP5) and the erythropoietin receptor (EPOR) relates to their role in conceptus erythropoiesis. ACP5, also known as uteroferrin, transports iron from the maternal endometrium to the developing porcine conceptus. Erythropoietin signaling through its receptor is critical to erythroid proliferation and differentiation. To further develop the porcine/human comparative map, and in the future identify genomic elements important to the expression of ACP5 and EPOR, large-insert genomic clones containing these genes were isolated from a porcine BAC library. One of the isolated BAC clones was found to contain both genes. Isolated clones were used to physically map the EPOR gene to SSC2, in close proximity to ACP5. A single BAC clone with an estimated 103 Kb genomic DNA insert was identified that contained both the EPOR and ACP5 genes. This BAC, as well as BACs containing EPOR or ACP5 exclusively, were subjected to restriction analysis and Southern hybridization. The EPOR probe hybridized to a ~ 14.5 Kb EcoR I fragment in BAC clones containing both genes or EPOR exclusively. The ACP5 probe hybridized to a ~13 Kb EcoR fragment in BAC clones containing both genes or ACP5 exclusively. This supports the specificity of the probe hybridizations and the close physical proximity of these two genes in BAC 324b2. To rule out the possibility that the proximity of these two genes in this BAC clone is due to chimerism, BAC clones containing EPOR or ACP5 exclusively were used as probes for FISH analysis. Both probes consistently hybridized to a single region of the porcine genome, SSC2q1-2-2.1. These results physically map the erythropoietin receptor gene to SSC2, in close proximity to ACP5.