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United States Department of Agriculture

Agricultural Research Service

Title: Development of Multiplex Sets of Simple Sequence Repeat DNA Markers in Soybean

Authors
item Narvel, James - IOWA STATE UNIVERSITY
item Chu, Wen-Chy - IOWA STATE UNIVERSITY
item Fehr, Walter - IOWA STATE UNIVERSITY
item Cregan, Perry
item Shoemaker, Randy

Submitted to: Molecular Breeding
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: August 20, 1999
Publication Date: N/A

Interpretive Summary: The use of molecular markers in plant breeding programs can speed the plant improvement process. However, using the markers can be very expensive. Any method that can cut the costs of using molecular markers in breeding programs will make it cheaper to produce improved plant varieties. In this research the scientists developed a method to analyze multiple markers at the same time. This results in a significant reduction in the cost of analyzing these markers. These technologies should now be more accessible to a wider range of research and soybean breeding programs which, in turn, will provide improved cultivars for the grower/consumer.

Technical Abstract: Multiplexing involves the analysis of several markers in a single gel lane that is based on the allele size range of marker loci. Multiplex SSR marker analysis is conducted with primers that are labeled with one of three dyes. The development of an SSR multiplex system requires estimates of the allele size range of markers to strategize primer labeling and for grouping markers into multiplex sets. A method is presented that describes the development of multiplex sets of SSR markers in soybean [Glycine max (L.) Merr.] by the selective placement of primer sites and by the analysis of diverse germplasm. Primer sites were placed at specific distances from the SSR to predispose the allele size range of marker loci. The analysis of pooled DNA samples comprising diverse soybean genotypes provided robust estimates of the allele size range of marker loci that enabled the development of multiplex sets. Eleven multiplex sets comprising 74 SSR markers distributed across the 20 linkage groups of soybean were developed. Multiplex sets constructed from the analysis of diverse soybean germplasm should have a wide range of genotyping applications. The procedures used in this study were systematic and rapid and should be applicable for multiplex development in any species with SSR marker technology.

Last Modified: 10/1/2014