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Title: SECOND COLLECTION OF UNIFORM, CARD GENERATED, VERTICALLY ELUTRIATED DUST FOR INTERLABORATORY COMPARISON OF DUST ENDOTOXIN ASSAYS

Author
item Chun, David
item Harrison, Robert
item Chew, Victor

Submitted to: Journal of Cotton Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/1/1999
Publication Date: N/A
Citation: Chun, D.T., Harrison, R.E., Chew, V. Second collection of uniform, card generated, vertically elutriated dust for interlaboratory comparison of dust endotoxin assays. Journal of Cotton Science. 1999. v.3 (4) 177-182.

Interpretive Summary: In an earlier work, dust samples on PVC filters were produced by an elaborate cotton blending and dust collection protocol. The dust samples produced were 'uniform', vertically elutriated dust samples. These dust samples were used in a two-part endotoxin assay study between different laboratories. The results from the study prompted the desire to continue the study. The supply of dust samples from the original dust collection was exhausted which meant that additional dust samples would have to be generated. To get the needed additional dust samples, cotton from 'three sources of cotton' was carded and the dust collected on two types of filters using vertical elutriators in the model cardroom at the Cotton Quality Research Station in Clemson, SC. Over 3,000 filter samples, each with 0.3 to 0.8-mg cotton dust, were collected which should satisfy the supply needs of the anticipated endotoxin assay study. This paper describes the cotton blending procedure and the dust collection process, and also a description of the dust sample population.

Technical Abstract: Previously, an elaborate cotton blending and dust collection protocol was developed and implemented to produce "uniform", vertically elutriated dust samples which were used in a two part interlaboratory endotoxin assay study. The results from that study generated interest in extending the interlaboratory endotoxin assay study. To satisfy this need, a second series of dust samples on polyvinyl chloride and glass filters were produced from 'three sources of cotton' using the model cardroom at the Cotton Quality Research Station in Clemson, SC. More than 1500 dust samples on polyvinyl chloride filters and 1500 dust samples on glass filters were collected which should satisfy need for dust samples in the upcoming endotoxin assay study. A description of the cotton blending and dust collection process will be presented as well as a description of the dust samples collected.