Submitted to: Animal Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: July 19, 1999
Publication Date: December 1, 1999
Citation: Sonstegard, T.S., Kappes, S.M. 1999. Mapping of the ucpi locus to bovine chromosome 17. Animal Genetics. 30(6):472.
Interpretive Summary: One approach to fine mapping potential quantitative trait loci (QTL's) is to develop the bovine comparative map. The current linkage map in cattle contains fewer than 300 gene loci, thus making comparisons with the gene rich mouse maps difficult. Development of the bovine map increases the desnity of markers associated with gene loci near the putative QTL, thereby allowing selection of positional candidate genes for further investigation. We used a PCR-based strategy to identify polymorphisms in non-coding regions of UCP1, presumed to be near a QTL for dairy form on bovine chromosome 27. Eight single nucleotide polymorphisms (SNPs) were detected in intron 1 of UCP1. Genotypic data generataed from a single SNP segregating in the MARC families was analyzed and integrated into the existing USDA/MARC linkage data set. The addition of UCP1 to the bovine genetic map improves the comparative map by reavealing new regions of conserved synteny between bovine chromosome 17 and mouse chromosome 8.
The bovine UCP1 cDNA (mitochondrial uncoupling protein 1) has been cloned and sequenced, however this gene locus has not been positioned on the genetic map. Primer pairs were designed to amplify across intron 1 for the purpose of detecting single nucleotide polymorphisms (SNPs). Sequence analysis of the UCP1 966 bp product derived from 12 parental animals of the eUSDA MARC reference map population revealed eight SNPs. Sense strand positions of the SNPs were C/T-186 (base types detected-nt position in 966 bpproduct), C/G-232, C/G-248, C/T-360, C/G-415, A/G-545, A/G-555, and C/T-588. Genotypes from the reference population were generated by PCR-RFLP detection of the T/C-186 SNP in the 832 bp UCP1 product. Linkage analysis positioned the UCP1 locus (23.5 cM) between EAF (twopoint rec. freq. = 0.05 LOD 7.16) and BMS2780 (two point rec. freq. = 0.03, LOD 9.13) on bovine chromosome 17 (BTA17). This addition of UCP1 to the bovine genetic map reveals new regions ofconserved synteny between BTA17 and MMU8. The human and swine orthologues of UCP1 are localized to HSA4q28-q31 and SSC8q21, respectively. Therefore the placement of bovine UCP1 agrees with conservation of synteny between BTA17q12-q23, HSA4q25-q31, and SSC8.