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United States Department of Agriculture

Agricultural Research Service

Title: Sequence Analysis of Resistance Gene Analogs from Potato

item Haynes, Kathleen
item Christ, Barbara - PENNSYLVANIA STATE UNIV.

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: May 12, 1999
Publication Date: N/A

Technical Abstract: Forty-two clones from a diploid Solanum phureja-S. stenotomum hybrid population were examined for lesion size and severity of foliage and tuber blight of Phytophthora infestans isolate US-8. This population is being used for molecular mapping studies in our laboratory. A detached leaf assay was used in assessing foliar resistance. The experimental design was a randomized complete block design with 3 replications. A treatment consisted of a terminal leaflet inoculated with a drop of sporangial suspension (approximately 1500 sporangia/drop) and incubated at 16C and >75% RH for 10 days. For assessing tuber resistance, tubers were inoculated by making an incision across the center of the tuber and placing approximately 2000 sporangia/tuber within the incision. Tubers were incubated in the dark at 12C and >90% RH for 12 days in plastic containers. A randomized complete block design with 6 replications was used. Lesion diameter and severity varied from 0 to 78 mm and 0 to 100% on foliage and 0 to 65 mm and 0 to 52.5% on tubers. Lesion diameter and severity differed significantly among the clones for both foliage and tuber resistance. The results showed that foliage and tuber blight resistance were not correlated.

Last Modified: 4/22/2015
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