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ARS Home » Research » Publications at this Location » Publication #100973
Title: INVESTIGATIONS INTO THE DEVELOPMENT OF CUCUMBER MOSAIC VIRUS AS VIRAL-BASEDVECTOR

Author
item Zhao, Yan
item TOUSIGNANT, MARIE - 1275-25-00
item Hammond, Rosemarie

Submitted to: American Society for Virology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Cucumber mosaic virus (CMV), the type member of the genus Cucumovirus, belongs to the family Bromoviridae, and has a divided genome consisting of three positive strand RNAs and a subgenomic RNA which is generated by transcription from RNA 3 and serves as the messenger RNA for the viral capsid protein. A CMV strain (CMV-Ix) originally isolated from Ixora had previously been cloned and the transcripts generated therefrom had been shown to be infectious. Development of CMV-Ix as a novel viral-based vector is based on trans-complementation by manipulating the genome component RNA 3. Preliminary results suggest that the engineered virus containing reporter genes GFP and GUS can move from cell to cell in the inoculated leaf and can enter the minor veins of the inoculated leaf but cannot move systemically. Derivatives of the vector are being evaluated for use as gene delivery systems for the expression of foreign gene products and for epitope display and vaccine production.