Technical Abstract: Most U.S. barley transformation efforts use the cultivar Golden Promise (GP) because its high rate of green plant regeneration makes recovery of transgenic plants relatively straightforward. Unfortunately, GP falls significantly short of current North American agronomic and malting quality requirements. Studies based on the responses of Morex and Harrington indicated that there were certain media component and culture technique modifications that significantly improved green plant regeneration from callus tissues of these two cultivars. The objective of this study was to determine whether these modifications were beneficial for a wide range of elite and modern Midwestern germplasm, and to identify potentially highly-regenerable lines for future transformation work. Culturing on improved media increased regeneration from each cultivar or line. All lines regenerated some green plants on improved media, including those 6-rowed lines that did not regenerate on standard media. Several single-plant-derived lines exhibited consistently high regeneration rates. Any of these lines would be a better starting point than GP from an agronomic perspective.