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United States Department of Agriculture

Agricultural Research Service

Title: Separate External and Internal Carcass Rinses from Eviscerated Broilers Orally Challenged with Salmonella

Authors
item Buhr, Richard
item Berrang, Mark
item Hinton, Jr, Arthur
item Ingram, Kimberly
item Rowland, G. - UNIVERSITY OF GEORGIA

Submitted to: Poultry Science Association Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: May 13, 1999
Publication Date: August 8, 1999
Citation: BUHR, R.J., BERRANG, M.E., HINTON JR, A., INGRAM, K.D., ROWLAND, G.N. SEPARATE EXTERNAL AND INTERNAL CARCASS RINSES FROM EVISCERATED BROILERS ORALLY CHALLENGED WITH SALMONELLA. POULTRY SCIENCE ASSOCIATION MEETING ABSTRACT. 1999.

Technical Abstract: Experiments were conducted to determine if the recovery of Salmonella from eviscerated broiler carcasses differed between the external and internal surfaces. Using a new evisceration-rinse technique, separate external and internal carcass rinses were made and compared for the positive-incidence of the marker Salmonella. At 5 wk of age, broilers were orally challenged with Salmonella Typhimurium and were processed five d later. Following electrocution, scalding, and defeathering, the neck of each broiler was tied to prevent leakage of crop contents, and the vent was sutured to prevent leakage of cloaca contents. The carcass was placed into a plastic bag to which 100 mL of sterile water was added. The carcass was shaken for 1 min and the external rinse collected. The crop and a cecum were collected aseptically and the remaining portions of the alimentary tract excised. The colon was tied within a latex glove to prevent leakage into the abdominal cavity. One hundred mL of sterile water was poured inside each carcass and the abdominal opening was sutured with a tight, continuous interlocking stitch. The carcass was shaken for 1 min and the internal carcass rinse aseptically collected. By direct plating, 0-15% of the external and internal rinses were positive for the marker Salmonella. Following enrichment, Salmonella was detected in 95% of external carcass rinses, 70% of internal carcass rinses, and 60% of the crops and ceca.

Last Modified: 7/25/2014