Submitted to: Poultry Science Association Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: May 13, 1999
Publication Date: August 8, 1999
Citation: BUHR, R.J., BERRANG, M.E., CASON JR, J.A., ROWLAND, G.N. PROCEDURE FOR OBTAINING SEPARATE EXTERNAL AND INTERNAL CARCASS RINSES FROM EVISCERATED BROILERS. POULTRY SCIENCE ASSOCIATION MEETING ABSTRACT. 1999.
A technique was developed to permit collection of separate external and internal rinses from defeathered carcasses. An abdominal incision was made from the keel to the vent, through which the alimentary tract from the crop to the ceca was excised. The colon was tied to prevent leakage through the vent and the neck was tied to prevent leakage through the thoracic inlet. One hundred mL of phosphate buffered saline (PBS) was poured inside each carcass and the abdominal opening was sutured with a tight continuous interlocking stitch. The carcass was then placed into a plastic bag to which 100 mL of PBS was added. To test the procedure, either the external or internal rinse for each carcass was spiked with nalidixic acid-resistant Salmonella Typhimurium. The carcass was then shaken for 1 min and the nonspiked rinse collected aseptically from either outside or inside the carcass. The marker Salmonella was not detected by direct plating for any carcass (level of detection 10 cfu per mL, n = 18) indicating very low level of migration of the marker between the external and internal rinse. After enrichment, however, 33% of nonspiked internal rinses and 92% of nonspiked external rinses contained the marker, indicating a greater rate of leakage of the spiked rinse from inside to outside had occurred. This exchange was circumvented by obtaining the outside carcass rinse prior to evisceration in subsequent experiments.