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United States Department of Agriculture

Agricultural Research Service

Title: Gene Mapping in Lentil with Recombinant Inbred Lines

Authors
item Tahir, M. - WA STATE UNIVERSITY
item Muehlbauer, Frederick

Submitted to: Journal of Heredity
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: February 4, 1994
Publication Date: N/A

Interpretive Summary: Gene maps of crop plants are valuable tools for crop improvement efforts because they show the location of the important genes and the linked markers. Those linked markers can be used for indirect selection in breeding and germplasm improvement programs. In this study, we used a population of recombinant inbred lines developed by single-seed-descent to develop a gene map of the lentil genome. The map is in good agreement with maps developed by other lentil mapping populations and similar linkage associations are shown. The information represents a valuable tool for improvement of lentil germplasm for traits of economic importance. The analysis of recombinant inbred lines of lentil provided additional information to further expand the genetic map.

Technical Abstract: Homozygous recombinant lines developed from interspecific Lens hybrids were used to map isozyme and morphological markers in lentil. F3 families from eight interspecific (L. culinaris x L. orientalis) lentil crosses were advanced to the F6 by single seed descent to develop eight sets of recombinant inbred lines. Each of the eight sets of lines was assayed for parental alleles at 2-8 isozyme and four morphological trait loci. Single locus goodness of fit to the expected 1:1 ratio was determined for each of the marker loci, and linkage relationships among the marker loci were determined. Six linkage groups were identified, and included a total of 17 isozyme loci and four morphological trait loci. We confirmed previously reported linkages among isozyme loci and morphological trait loci (Gs: Aat-p, Pi:Gal-1, Yc:Pgm-p, Aat-c:Me-2, Aat-mb:Skdh, Fk:Pgd-p), and identified three additional linkage groups (Gh:Gal-1, Gal-2:Aat-mb:Skdh, and Skdh:Aldo:Nag). The genetic map of lentil was expanded and confirmed based on these linkages, and a database was generated that can be used to add additional markers to the gene map.

Last Modified: 7/28/2014
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