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United States Department of Agriculture

Agricultural Research Service

Title: Preliminary Results of Nir Spectroscopy to Determine Starch and Protein Quality in Small Samples. (Poster Presentation at Aacc Meeting)

item Dowell, Floyd
item Rowley, N - KSU, MANHATTAN, KS 66506
item Xie, F - KSU, MANHATTAN, KS 66506
item Tilley, Michael
item Bean, S - KSU, MANHATTAN, KS 66506
item Seib, P - KSU, MANHATTAN, KS 66506
item Lookhart, George

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: July 28, 1999
Publication Date: October 31, 1999
Citation: Dowell, F.E., Rowley, N.K., Xie, F., Tilley, M., Bean, S.R., Seib, P.A., Lookhart, G.L. 1999. Preliminary results of nir spectroscopy to determine starch and protein quality in small samples. Abstract No. 83 in: 1999 AACC Annual Meeting Program Book. p.172. Meeting Abstract.

Technical Abstract: Most NIR instruments are designed to measure absorption or reflection characteristics of bulk samples. However, there are many instances when large amounts of material are not available, such as when analyzing breeder samples or other small-scale testing. In these instances, NIR analysis of a single kernel, or a portion of flour from a single kernel, may be desired. Procedures were developed utilizing fiber optics attached to a Perten Instrument DA7000 spectrometer (Springfield, Illinois) to collect absorbance spectra from these ultra-small samples. Results from testing small samples (<20 mg) to determine waxy character (related to the presence or absence of GBSS proteins), amount of gliadin, amount of glutenin, and other quality attributes will be presented. The procedure is rapid (<1 s), non-destructive, and requires little sample preparation.

Last Modified: 4/19/2015
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