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United States Department of Agriculture

Agricultural Research Service

Title: Nematode-Antagonistic Activity of Gliocladium Virens and Burkholderia Cepacia

Authors
item Meyer, Susan
item Roberts, Daniel
item Massoud, Samia - SUEZ CANAL UNIVERSITY
item Chitwood, David

Submitted to: Society of Nematology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: July 6, 1999
Publication Date: N/A

Technical Abstract: The fungus Gliocladium virens and the bacterium Burkholderia cepacia were studied as potential microbial control agents for Meloidogyne incognita on tomato, and as producers of natural compounds that could be efficacious for decreasing nematode populations. In assays conducted in microwell tissue culture plates, filtered culture broth from B. cepacia grown in potato dextrose broth (PDB) reduced M. incognita egg hatch ca. 50%. Culture filtrate from G. virens grown in PDB did not reduce egg hatch, but did affect mobility of hatched second-stage juveniles (J2), tripling the number of immobile J2 compared with a PDB control. In an initial trial with G. virens applied as a root drench to growth chamber-grown tomato plants, nematode population numbers (eggs and J2) counted 35 days after addition of M. incognita were reduced ca. 40%. However, in a greenhouse trial with G. virens applied as a seed treatment and again as a root drench to young tomato plants, nematode population numbers at 35 days were not affected by application of G. virens. Burkholderia cepacia was also studied in the greenhouse trial (applied to tomato as a seed coat and as a root drench); application resulted in ca. 41% reduction in nematode population numbers.

Last Modified: 11/20/2014
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