"Characterization of the immune reagent chicken IL-16"

Authors: GRANT, AR'QUETTE - US Department Of Agriculture (USDA); KIM, WOO - US Department Of Agriculture (USDA); Lillehoj, Hyun

Submitted to: BARC Poster Day
Publication Type: Other
Publication Acceptance Date: 4/7/2018
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Interleukin-16 has been characterized as a pro-inflammatory cytokine that mediates an immune response in human and mouse monocytes and peripheral blood mononuclear cells (PBMC), and it plays a role in proliferating B-cells and myelomas. The function of chicken IL-16 ortholog (ch-IL-16) is far less understood and only shares 52% and 49% sequence homology with human and mouse, respectively. Although it has previously shown chemotaxis abilities in chicken splenocytes, the low sequence homology to their mammalian counterparts, the current study was undertaken to characterize the function of yeast-expressed ch-IL-16 as an immune reagent in pro-inflammatory cytokine production and cell proliferation. In a dose dependent manner, ch-IL-16 was applied to 5 x 106 cells/mL PBMC and HD11 chicken monocytes, and the extracted RNA was used to determine the pro-inflammatory cytokine production. Also in a dose dependent manner, ch-IL-16 was applied to primary bursal lymphocytes, PBMC, and RP-9 chicken myeloma cells. Ch-IL-16 produced pro-inflammatory cytokines: IL-6, IL-1ß, and TL1A which is the chicken equivalent to TN1-a at an optimal concentration between 40 and 50 ng/mL. Further characterization efforts include analyzing the proliferation effects of ch-IL-16 on bursal lymphocytes, PBMC and RP-9. Future directions also include the production of monoclonal antibodies to neutralize the function of ch-IL-16. These results would be useful in the characterization of the chicken immune system, and the role of ch-IL-16 as a mediatory cytokine.