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ARS Home » Southeast Area » New Orleans, Louisiana » Southern Regional Research Center » Food and Feed Safety Research » Research » Publications at this Location » Publication #345732
Research Project: Molecular and Environmental Factors Controlling Aflatoxin Reduction by Non-Toxigenic Aspergillus Strains

Location: Food and Feed Safety Research

Title: Aspergillus korhogoensis, a novel aflatoxin producing species from the Côte d’Ivoire

Author
item CARVAJAL-CAMPOS, AMARANTA - Toxalim, Research Centre In Food Toxicology
item MANIZAN, AMA - Université Nangui Abrogoua
item TADRIST, SOURIA - Toxalim, Research Centre In Food Toxicology
item AKAKI, DAVID - Institut National Polytechnique Félix Houphouët-Boigny (INP-HB)
item KOFFI-NEVRY, ROSE - Université Nangui Abrogoua
item Moore, Geromy
item FAPOHUNDA, STEPHEN - Babcock University
item BAILLY, SYLVIANE - Toxalim, Research Centre In Food Toxicology
item MONTET, DIDIER - Centro De Cooperation Internationale En Recherche Agronomique Pour Le Development (CIRAD)
item OSWALD, ISABELLE - Toxalim, Research Centre In Food Toxicology
item LORBER, SOPHIE - Toxalim, Research Centre In Food Toxicology
item BRABET, CATHERINE - Centro De Cooperation Internationale En Recherche Agronomique Pour Le Development (CIRAD)
item PUEL, OLIVIER - Toxalim, Research Centre In Food Toxicology

Submitted to: Toxins
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/25/2017
Publication Date: 10/31/2017
Citation: Carvajal-Campos, A., Manizan, A.L., Tadrist, S., Akaki, D.K., Koffi-Nevry, R., Moore, G.G., Fapohunda, S.O., Bailly, S., Montet, D., Oswald, I.P., Lorber, S., Brabet, C., Puel, O. 2017. Aspergillus korhogoensis, a novel aflatoxin producing species from the Côte d’Ivoire. Toxins. 9:353. https://doi.org/10.3390/toxins9110353.
DOI: https://doi.org/10.3390/toxins9110353

Interpretive Summary: Several strains of a new aflatoxigenic species of Aspergillus, A. korhogoensis, were isolated in the course of a screening study involving species from section Flavi found contaminating peanuts (Arachis spp.) and peanut paste in the Ivory Coast. This species is capable of producing B- and G- aflatoxins, as well as small sclerotia similar to several other recently described species in section Flavi. A. korhogoensis exhibits similar morphological, metabolic and physiological characteristics to multiple closely-related species, but molecular examination involving sequences from nine genomic regions showed this to be a novel species.

Technical Abstract: Based on examination of four isolates, this new species is described using a polyphasic approach. A concatenated alignment comprised of nine genes (ITS, benA, cmdA, mcm7, amdS, rpb1, preB, ppgA, preA) was subjected to phylogenetic analysis, and resulted in all four strains being inferred as a distinct clade. Characterization of mating type for each strain revealed A. korhogoensis as a heterothallic species, since three isolates exhibited a singular MAT1-1 locus and one isolate exhibited a singular MAT1-2 locus. Morphological and physiological characterizations were also performed based on their growth on various types of media. Their respective extrolite profiles were characterized using LC/HRMS, and showed that this new species is capable of producing B- and G-aflatoxins, aspergillic acid, and CPA, as well as other metabolites. Altogether, our results confirm the monophyly of A. korhogoensis, and strengthen its position in the A. flavus clade, as the sister taxon of A. parvisclerotigenus.