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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » Hard Winter Wheat Genetics Research » Research » Publications at this Location » Publication #345053
Title: Genetic variations of HvP5CS1 and their association with drought tolerance related traits in barley (Hordeum vulgare L.)

Author
item XIA, YANSHI - Guangdong Academy Of Agricultural Sciences
item LI, RONGHUA - Guangzhou University
item Bai, Guihua
item SIDDIQUE, KADAMBOT H.M. - University Of Western Australia
item VARSHNEY, RAJEEV - International Crops Research Institute For Semi-Arid Tropics (ICRISAT) - India
item BAUM, MICHAEL - International Center For Agricultural Research In The Dry Areas (ICARDA)
item YAN, GUIJUN - University Of Western Australia
item GUO, PEIGUO - Guangzhou University

Submitted to: Scientific Reports
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/10/2017
Publication Date: 8/11/2017
Citation: Xia, Y., Li, R., Bai, G., Siddique, K., Varshney, R., Baum, M., Yan, G., Guo, P. 2017. Genetic variations of HvP5CS1 and their association with drought tolerance related traits in barley (Hordeum vulgare L.). Scientific Reports. 7:7870. https://doi.org/10.1038/s41598-017-08393-0.
DOI: https://doi.org/10.1038/s41598-017-08393-0

Interpretive Summary: The accumulation of proline is correlated with increased drought stress tolerance in many plant species. Delta-1-pyrroline-5-carboxylate synthase is an enzyme involved in the synthesis of proline. The gene encoding this enzyme is called P5CS1 and is induced by drought stress in barley. Based on DNA sequencing, we identified 13 distinct variants of P5CS1 in 287 barley accessions collected worldwide. Five of the sequence variants were significantly associated with improved drought tolerance traits and two sequence variations were significantly associated with increased grain yield. The sequence variations associated with drought tolerance-related traits are potential markers for selecting improved drought tolerance in barley.

Technical Abstract: Delta-1-pyrroline-5-carboxylate synthase gene1 (P5CS1) is the key gene involved in the biosynthesis of proline and is significantly induced by drought stress. The exploration of genetic variation in HvP5CS1 may facilitate a better understanding of the mechanism of drought adaptation in barley. In the current study, 41 polymorphisms including 16 single nucleotide polymorphisms (SNPs) and 25 insertions/deletions (INDELs) were detected in HvP5CS1 among 287 barley (Hordeum vulgare L.) accessions collected worldwide, with 13 distinct haplotypes identified in the barley collection. Five polymorphisms in HvP5CS1 were significantly (P < 0.001) associated with drought tolerance related traits in barley. The phenotypic variation of a given trait explained by each associated polymorphism ranged from 4.43% to 9.81%. Two sequence variations that were significantly (P<0.0001) associated with grain yield had marginally significant positive Tajima’s D values in the sliding window, so they might have been selected for environmental adaptation. Meanwhile, two haplotypes HvP5CS1_H1 and HvP5CS1_H4, which contained desired alleles of the two variations mentioned above, were significantly (P < 0.001) associated with drought tolerance related traits, and explained 5.00~11.89% of the phenotypic variations. These variations associated with drought tolerance related traits can be used as potential markers for improving drought tolerance in barley.