Evaluating the microbiome of two sampling locations in the nasal cavity of cattle with bovine respiratory disease complex (BRDC)

Authors: McDaneld, Tara; Kuehn, Larry; Keele, John

Submitted to: Journal of Animal Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/14/2018
Publication Date: 5/22/2018
Publication URL: http://handle.nal.usda.gov/10113/6472291
Citation: McDaneld, T.G., Kuehn, L.A., Keele, J.W. 2018. Evaluating the microbiome of two sampling locations in the nasal cavity of cattle with bovine respiratory disease complex (BRDC). Journal of Animal Science. 96:1281-1287. https://doi.org/10.1093/jas/sky032.
DOI: https://doi.org/10.1093/jas/sky032

Interpretive Summary: Bovine respiratory disease complex (BRDC) is an involved multi-factor disease, which is the most expensive animal disease afflicting herds in U.S. beef cattle industry, costing the industry over $1 billion annually. Animals may be predisposed to suffer from BRDC by a variety of bacterial agents that comprise the animal’s bacterial populations of the nasal cavity. Common sampling techniques of the nasal cavity utilize expensive sampling swabs and require additional restraint of the animal to complete sampling. Therefore, there is a need to identify alternative sampling techniques to evaluate bacterial populations in the nasal cavity. In this study, we identified the bacterial pathogens in the upper nasal cavity, using two common sampling techniques, of cattle diagnosed with BRDC and healthy cohorts. The two sampling techniques were a 6-inch nasal swab and an 8-inch nasal swab. Overall, we were able to demonstrate that bacterial pathogens are similar between sampling sites for both common sampling techniques of the nasal cavity. Together, these data indicate that the less invasive 6-inch nasal swab can be used to study the upper respiratory bacterial pathogens in relation to calves diagnosed with BRDC for lower cost and inflicting less stress on animals.

Technical Abstract: Bovine respiratory disease complex (BRDC) is a multifactor disease, and disease incidence may be associated with an animal’s commensal bacterial populations (microbiome) in the upper nasal cavity. Identifying these commensal bacterial populations in the upper nasal cavity may help us to understand the impact of the microbiome on incidence of BRDC in cattle. Various sampling techniques have previously been utilized to evaluate the microbiome of different locations of the upper nasal cavity in cattle. Therefore, our objective was to determine whether bacterial populations of the nasal cavity vary based on these sampling locations. Two common sampling techniques were evaluated, including 6-inch nasal swabs and deep nasopharyngeal swabs. Nasal swabs from calves were collected when the animal was diagnosed with BRDC after weaning in the feedlot in addition to collection of samples from asymptomatic cohorts. Samples were pooled in groups based on year the animal was in the feedlot (2015 or 2016), when the animal was diagnosed with BRDC (1 to 5 weeks after weaning), type of sample (6-inch nasal swab or deep nasopharyngeal swab), and health status (diagnosis with BRDC or control). Variable regions 1 through 3 along the 16S rRNA gene were amplified by PCR and sequenced using next-generation sequencing (Illumina MiSeq) for identification of the bacterial taxa present. Overall, sampling site did not consistently influence diversity of the bacterial populations of the upper nasal cavity. However, the effect of disease incidence on the microbiome was depended on sampling time after weaning (P = 0.0462) for 2015, while the main effects of sampling time after weaning (P = 0.00992) and disease phenotype (P = 0.012) were significant for 2016. These data for 2016 demonstrate that in addition to bacterial profiles changing throughout weaning, calves diagnosed with BRDC have different bacterial profiles compared to their control cohorts. In addition, evaluation of the microbiome identified predominant bacteria genera in the upper nasal cavity included those previously reported to be associated with cattle diagnosed with BRDC including Mycoplasma sp., Psychrobacter sp., and Mannheimia sp. In summary, these results demonstrate that shorter, less invasive 6-inch nasal swabs produce similar results to deep nasopharyngeal swabs.