Structures of hydroxy fatty acids as the constituents of triacylglycerols in Philippine wild edible mushroom, Ganoderma lucidum

Authors: Lin, Jiann; Hou, Ching; DULAY, RICH - Central Luzon State University; Ray, Karen; Chen, Grace

Submitted to: Biocatalysis and Agricultural Biotechnology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/23/2017
Publication Date: 10/9/2017
Citation: Lin, J.T., Hou, C.T., Dulay, R.M., Ray, K.J., Chen, G.Q. 2017. Structures of hydroxy fatty acids as the constituents of triacylglycerols in Philippine wild edible mushroom, Ganoderma lucidum. Biocatalysis and Agricultural Biotechnology. 12/148-151. https://doi.org/10.1016/j.bcab.2017.09.010.
DOI: https://doi.org/10.1016/j.bcab.2017.09.010

Interpretive Summary: Some Philippine wild edible mushrooms have been shown to have many health benefits. We have recently reported the identification and quantification of seventy-seven molecular species of acylglycerols containing hydroxy fatty acids (HFA) in mushroom, Ganoderma lucidum. We reported the masses of HFA and the numbers of hydroxyl groups and double bonds of the HFA. The high contents of HFA and the presence of HFA of odd numbered carbon atoms in this Philippine mushroom might be related to one or more of its health benefits. We report here the structures of some HFA in this Philippine mushroom including the HFA chain lengths, locations of hydroxyl groups and double bonds on the fatty acid chains. The structures of these HFA in mushroom are the same or similar to those in castor oil, except the presence of HFA with odd numbered carbon atoms.

Technical Abstract: Edible Philippine mushrooms including Ganoderma lucidum have many health benefits. We have recently reported the identities and the contents of 77 molecular species of acylglycerols containing hydroxy fatty acids (HFA) in this mushroom. The structures of these HFA were proposed using the electrospray ionization mass spectrometry of the lithium adducts of acylglycerols in the HPLC fractions of the lipid extract. The proposed structures of the HFA were OH1319:110, OH1319:114, 2OH11,1218:0, 2OH11,1218:19, 2OH11,1218:29,13, 3OH11,12,1318:19 and 3OH11,12,1318:114. The locations of the hydroxyl groups and double bonds were near the middle of the HFA chains. The structures were the same or similar to that of HFA in castor oil except that the HFA of odd numbered carbon atoms were not detected in castor oil. OH18:1 was identified in the mushroom and its structure was not OH1218:19 (ricinoleate) as that in castor oil. However, ricinoleate seems to be the biological precursor of HFA in the mushroom as that in castor structurally.