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ARS Home » Southeast Area » Stoneville, Mississippi » Warmwater Aquaculture Research Unit » Research » Publications at this Location » Publication #342839

Title: Ovarian fragment sizes affect viability and morphology of preantral follicles during storage at 4°C

Author
item GASTAL, GUSTAVO - Southern Illinois University
item ALVES, BENNER - Southern Illinois University
item ALVES, KELE - Southern Illinois University
item SOUZA, M - Southern Illinois University
item VIEIRA, D - Universidade Estadual Do Ceara
item VARELA JR, S - Universidade Federal De Pelotas
item FIGUEIREDO, RICARDO - Universidade Estadual Do Ceara
item FEUGANG, JEAN - Mississippi State University
item LUCIA JR, T - Universidade Federal De Pelotas
item GASTAL, EDUARDO - Southern Illinois University

Submitted to: Reproduction
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/28/2017
Publication Date: 2/28/2017
Citation: Gastal, G.A., Alves, B.G., Alves, K.A., Souza, M.E., Vieira, D.A., Varela Jr, S.A., Figueiredo, R.J., Feugang, J.M., Lucia Jr, T., Gastal, E.L. 2017. Ovarian fragment sizes affect viability and morphology of preantral follicles during storage at 4°C. Reproduction. 153:577-587.

Interpretive Summary: Ovarian preservation is another way to restore fertility of females undergoing critical conditions or death. However, the production of mature viable gametes after in vitro culture or transplantation of ovarian grafts are still challenging. Here evaluated the survivability of various sampling of mare ovaries and storage time at 4oC. Results indicated better survivability of biopsy size fragments following long storage to up of 24 hours post collection. Findings provides new data to efficient transportation of viable ovarian tissues from donors to beneficiaries.

Technical Abstract: The efficient transportation of ovarian tissues is affected b various factors compromising their viability. We tested various ovarian sample sizes (whole ovary, biopsy, and transplantation size) during various transportation times.