Author
GASTAL, GUSTAVO - Southern Illinois University | |
ALVES, BENNER - Southern Illinois University | |
ALVES, KELE - Southern Illinois University | |
SOUZA, M - Southern Illinois University | |
VIEIRA, D - Universidade Estadual Do Ceara | |
VARELA JR, S - Universidade Federal De Pelotas | |
FIGUEIREDO, RICARDO - Universidade Estadual Do Ceara | |
FEUGANG, JEAN - Mississippi State University | |
LUCIA JR, T - Universidade Federal De Pelotas | |
GASTAL, EDUARDO - Southern Illinois University |
Submitted to: Reproduction
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 2/28/2017 Publication Date: 2/28/2017 Citation: Gastal, G.A., Alves, B.G., Alves, K.A., Souza, M.E., Vieira, D.A., Varela Jr, S.A., Figueiredo, R.J., Feugang, J.M., Lucia Jr, T., Gastal, E.L. 2017. Ovarian fragment sizes affect viability and morphology of preantral follicles during storage at 4°C. Reproduction. 153:577-587. Interpretive Summary: Ovarian preservation is another way to restore fertility of females undergoing critical conditions or death. However, the production of mature viable gametes after in vitro culture or transplantation of ovarian grafts are still challenging. Here evaluated the survivability of various sampling of mare ovaries and storage time at 4oC. Results indicated better survivability of biopsy size fragments following long storage to up of 24 hours post collection. Findings provides new data to efficient transportation of viable ovarian tissues from donors to beneficiaries. Technical Abstract: The efficient transportation of ovarian tissues is affected b various factors compromising their viability. We tested various ovarian sample sizes (whole ovary, biopsy, and transplantation size) during various transportation times. |