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ARS Home » Northeast Area » Washington, D.C. » National Arboretum » Floral and Nursery Plants Research » Research » Publications at this Location » Publication #340730
Title: Generation of an infectious clone of a new Korean isolate of apple chlorotic leaf spot virus driven by dual 35S and T7 promoters in a versatile binary vector

Author
item KIM, IK-HYUN - Chungnam National University
item HAN, JAE-YEONG - Chungnam National University
item CHO, IN-SOOK - National Institute Of Horticultural & Herbal Science (NIHHS)
item MOON, JAE-SUN - University Of Science And Technology Of China
item SEO, EUN-YOUNG - Chungnam National University
item KIM, HONG-GI - Chungnam National University
item Hammond, John
item LIM, HYOUN-SUB - Chungnam National University

Submitted to: Plant Pathology Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/8/2017
Publication Date: 12/12/2017
Citation: Kim, I., Han, J., Cho, I., Moon, J., Seo, E., Kim, H., Hammond, J., Lim, H. 2017. Generation of an infectious clone of a new Korean isolate of apple chlorotic leaf spot virus driven by dual 35S and T7 promoters in a versatile binary vector. Plant Pathology Journal . 33(6):608-613. https://doi.org/10.5423/PPJ.NT.05.2017.0106.
DOI: https://doi.org/10.5423/PPJ.NT.05.2017.0106

Interpretive Summary: Virus infections cause reductions in yield and quality of many crops. Vegetatively-propagated fruit trees are prone to accumulation of viruses that are transmitted either mechanically or via vector transmission in the course of crop production. Some tree-fruit viruses produce few visible symptoms, but may cause significant yield losses. Apple chlorotic leaf spot virus (ACLSV) causes minimal symptoms in many apple cultivars, but is known to cause yield losses of 30-40%, and was recently identified in Korea. We found that the Korean isolate of ACLSV was distinct from previously characterized isolates from other countries, and developed an infectious clone of this isolate to screen Korean apple breeding stocks for tolerance to ACLSV. This will be valuable for use in selecting apple breeding stocks with tolerance or resistance to infection by this Korean isolate, and will therefore improve yield potential.

Technical Abstract: The full-length sequence of a new isolate of Apple chlorotic leaf spot virus (ACLSV) from Korea was divergent, but most closely related to the Japanese isolate A4, at 84% nucleotide identity. The full-length cDNA of the Korean isolate of ACLSV was cloned into a binary vector downstream of the bacteriophage T7 RNA promoter and the Cauliflower mosaic virus 35S promoter. Chenopodium quinoa was successfully infected using in vitro transcripts synthesized using the T7 promoter, detected at 20 days post inoculation (dpi), but did not produce obvious symptoms. Nicotiana occidentalis and C. quinoa were inoculated through Agroinfiltration. At 32 dpi the infection rate was evaluated; no C. quinoa plants were infected by agroinfiltration, but infection of N. occidentalis was obtained.