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ARS Home » Southeast Area » Booneville, Arkansas » Dale Bumpers Small Farms Research Center » Research » Publications at this Location » Publication #327289
Title: A triple gene mutant of BoHV-1 administered intranasally in lambs replicates efficiently in the nasal epithelium and induces neutralizing antibody

Author
item LAY, CHRISTIAN - Louisiana State University
item Burke, Joan
item PAULSEN, DANIEL - Louisiana State University
item CHOWDHURY, SHAFIQUL - Louisiana State University

Submitted to: International Journal of Vaccines and Immunization
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/25/2016
Publication Date: 1/29/2016
Citation: Lay, C.T., Burke, J.M., Paulsen, D.B., Chowdhury, S.I. 2016. A triple gene mutant of BoHV-1 administered intranasally in lambs replicates efficiently in the nasal epithelium and induces neutralizing antibody. International Journal of Vaccines and Immunization. 2(1):1-4.

Interpretive Summary: Bovine herpes virus 1 (BoHV-1) causes respiratory infections and abortions in cattle, and is an important component of Bovine respiratory disease complex (BRDC). BoHV-1 has also been isolated from sheep with respiratory disorder. Scientists at Louisiana State University and USDA, Agricultural Research Service, Booneville, AR were able to engineer a mutated BoHV-1 vaccine and intranasally inoculate lambs, stimulating an immune response. The mutated BoHV-1 has the potential as a vaccine against BoHV-1 in livestock and as a viral vector for protective antigens of other bovine respiratory viruses. Results are important for future research in livestock health and has impact to human health, possibly extending our work to develop vaccines against Rift Valley Fever virus in humans and livestock.

Technical Abstract: Bovine herpes virus 1 (BoHV-1) causes respiratory infections and abortions in cattle, and is an important component of Bovine respiratory disease complex (BRDC). BoHV-1 has also been isolated from sheep with respiratory disorder. Experimentally, sheep and goats are infected productively with BoHV-1 after intranasal inoculation and they generate immune response against BoHV-1. We have engineered a triple gene- mutated bovine herpes virus type 1 (BoHV-1 tmv) vaccine virus which is protective against BoHV-1 in calves. In the present study, we have tested BoHV-1 tmv infectivity and serum neutralizing antibody titers in lambs after intranasal inoculation. The results showed that BoHV-1 tmv replicated well in the nasal mucosa with moderate titers for 7 days and the infected lambs generated serum neutralizing titers.