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ARS Home » Pacific West Area » Kimberly, Idaho » Northwest Irrigation and Soils Research » Research » Publications at this Location » Publication #319376

Title: Identification of differentially expressed genes induced by beet curly top virus infection in sugarbeet

Author
item Eujayl, Imad
item Strausbaugh, Carl

Submitted to: Journal of Sugar Beet Research
Publication Type: Abstract Only
Publication Acceptance Date: 2/26/2015
Publication Date: 7/28/2015
Citation: Eujayl, I.A., Strausbaugh, C.A. 2015. Identification of differentially expressed genes induced by beet curly top virus infection in sugarbeet. Journal of Sugar Beet Research. 52(1&2):98-99.

Interpretive Summary:

Technical Abstract: Resistance to beet curly top virus (BCTV) trait is crucial in Western USA. There is sparse public knowledge of genes regulating resistance. This research focused on gene expression profiling of resistance to the three BCTV strains: Cal/Logan (Cal), Worland (Wor), and severe. Differential gene expression was studied via RNA-sequencing of a highly resistant doubled haploid line (KDH13-PI663862) compared to a highly susceptible line (K19-19). KDH13 was subjected to 7 treatments: infested with non-infectious leafhoppers, infections with leafhoppers population carrying a single, two, or three strains, and control leaf transcriptome, and K19-19 infected with the three strains. RNA was extracted from leaves of the 8 treatments (3 replications, 24 mRNA libraries), sequenced in a HiSeq2500 and analyzed using TopHat and Cufflinks software. All sequences were aligned to the RefBeet-1.2. Based on 28 pair-wise comparisons, differentially expressed (DE) genes were determined with an adjusted false-discovery-rate (q value) lower than 0.05. For example, DND1 gene, known to be involved in innate immune response to pathogens (6.8kb located on chromosome1), was found down regulated (q = 0.01), in KDH13 when infected with the three strains compared to infestation with non-infections leafhoppers, but no response of DND1 was observed in K19-19 infected with the three strains. There was a pattern of some defense gene families, including ERF genes subunits that were up-regulated when KDH13 was infected with Wor only and down-regulated when its infected with both Cal and Wor, and evidence of strain-specific and strain interaction gene expression. This research is the first to reveal gene transcriptional profiles associated with resistance as well as susceptibility to BCTV.