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ARS Home » Southeast Area » Stuttgart, Arkansas » Dale Bumpers National Rice Research Center » Research » Publications at this Location » Publication #318234
Title: In vitro fermentation patterns of rice bran components by human gut microbiota

Author
item PHAM, TUNG - University Of Arkansas
item SAVARY, BRETT - Arkansas State University
item Chen, Ming Hsuan
item LEE, SUN-OK - University Of Arkansas

Submitted to: Symposium Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 6/18/2015
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Rice bran is a rich source of bioactive components that can promote gastrointestinal health. However, bran is removed during polishing. Among those, feruloylated arabinoxylan oligosaccharides (FAXO) and rice bran polyphenolics (RBPP) are hypothesized to have positive impacts on human gut microbiota as prebiotics. In this study, FAXO and 50% ethanol fraction of RBPP, were assessed for their ability to increase short-chain fatty acids (SCFA) production, including acetic, propionic, and butyric acid, which are strongly associated with colonic health in human. Fresh fecal samples collected from healthy adults with no signs or symptoms of bowel diseases or conditions were diluted with anaerobic medium. Each sample received 5 treatments separately: Blank (no substrates), Control-FOS (fructooligosaccharides), FAXO, RBPP, and FAXO with RBPP. Samples were prepared inside an anaerobic chamber, then capped and sealed tightly. Samples were incubated at 37oC. An aliquot of 1.5 mL was drawn from each test tube at 0, 4, 8, 12, 24, and 30 hours and stored immediately at -80oC. SCFA production was assessed using gas chromatography. Analyzed data from 3 subjects showed that SCFA productions, particularly acetic acid and butyric acid, were significantly increased with the addition of FAXO (P<0.05). The synergistic effects of FAXO and RBPP were also observed as their combination increase the SCFA production at several time points (P<0.05). Results from this study suggest the prebiotic properties of FAXO and its positive interaction with RBPP isolated from rice bran.