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ARS Home » Southeast Area » Charleston, South Carolina » Vegetable Research » Research » Publications at this Location » Publication #317894
Title: Complete genome sequence of an emerging melon necrotic spot virus isolate infecting greenhouse cucumber in North America

Author
item Li, Rugang
item ZHENG, YI - Boyce Thompson Institute
item FEI, ZHANGJUN - Boyce Thompson Institute
item Ling, Kai-Shu

Submitted to: Genome Announcements
Publication Type: Research Notes
Publication Acceptance Date: 6/15/2015
Publication Date: 7/16/2015
Citation: Li, R., Zheng, Y., Fei, Z., Ling, K. 2015. Complete genome sequence of an emerging melon necrotic spot virus isolate infecting greenhouse cucumber in North America. Genome Announcements. 3(4):e00775-15.

Interpretive Summary: The complete genome sequence of an emerging Melon necrotic spot virus isolate infecting greenhouse cucumber in Canada was determined. MNSV infects mainly the species of the cucurbits including melon, cucumber, and watermelon in the field, causing serious yield loss. The virus is seed-borne and is widely distributed in Asia and Europe. The close phylogenetic relationship to the European isolates of MNSV suggests that this Canadian isolate might have been introduced in seeds from Europe.

Technical Abstract: The complete genome (4,267 nt) of an Melon necrotic spot virus (MNSV) isolate (ABCA13-01) infecting greenhouse cucumber in Canada was determined through deep sequencing of small RNAs. Its genome sequence was most closely related to MNSV-N (97%), but lacking a 55-nt insertion at the 3’UTR for resistance breaking.