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Title: Identification and evaluation of SNP and SCAR markers linked to the Rychc gene for resistance to Potato virus Y in diploid potato populations

Author
item FULLADOLSA, ANA - University Of Wisconsin
item Jansky, Shelley
item Halterman, Dennis
item CHARKOWSKI, AMY - University Of Wisconsin

Submitted to: American Phytopathological Society
Publication Type: Abstract Only
Publication Acceptance Date: 6/4/2015
Publication Date: 8/1/2015
Citation: Fulladolsa, A.C., Jansky, S.H., Halterman, D.A., Charkowski, A. 2015. Identification and evaluation of SNP and SCAR markers linked to the Rychc gene for resistance to Potato virus Y in diploid potato populations [abstract]. American Phytopathological Society. Paper No. 328-P.

Interpretive Summary:

Technical Abstract: Potato virus Y (PVY) is the most significant cause of economic loss due to plant pathogens in seed potato production. With the emergence of PVY strains and infection phenotypes in the last decade, the need for varieties with broad resistance has increased in North America. Extreme resistance to PVY has been attributed to single-dominant genes found in wild relatives of potato. Two accessions of Solanum chacoense, carrying the PVY resistance gene Rychc, were used to develop segregating diploid populations. The phenotypes of 175 individuals of a population derived from an accession heterozygous for Rychc showed a 1:1 resistance segregation ratio. We used the SolCAP 8303 SNP Infinium array to genotype the population and identified five SNPs correlating with resistance, located on the distal end of chromosome IX. One SNP was selected to design a TaqMan SNP genotyping assay. We also sequenced the genome of the other S. chacoense accession, homozygous for Rychc, from which a useful breeding clone, XD3, was selected and selfed. We used the genome sequence of the parental S. chacoense to develop a co-dominant SCAR marker. Initial screening of our populations with the TaqMan assays shows that it is effective for SNP identification, but the SNPs associated with resistance vary between populations derived from different parents. Similarly, the SCAR marker is useful for genotyping the XD3-selfed population, but not for others.