Skip to main content
ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Genetics and Animal Breeding » Research » Publications at this Location » Publication #313793
Title: Design of a DNA panel for genomic studies in Russian cattle breeds

Author
item YUDIN, NIKOLAY - Russian Academy Of Sciences
item VASIL'EVA, L - Russian Academy Of Sciences
item BELYAVSKAYA, V - State Research Of Virology And Biotechnology (VECTOR INSTITUTE)
item AITNAZAROV, R - Russian Academy Of Sciences
item SMIRNOV, P - Novosibirsk State University
item Heaton, Michael - Mike
item LAEGREID, WILLIAM - University Of Wyoming
item ORLOWA, G - Russian Academy Of Sciences
item ROMASHENKO, A - Russian Academy Of Sciences
item VOEVODA, MIKHAIL - Russian Academy Of Sciences

Submitted to: Russian Journal of Genetics: Applied Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/15/2015
Publication Date: 2/18/2015
Citation: Yudin, N.S., Vasil'Eva, L.A., Belyavskaya, V.A., Aitnazarov, R.B., Smirnov, P.N., Heaton, M.P., Laegreid, W.W., Orlowa, G.V., Romashenko, A.G., Voevoda, M.I. 2015. Design of a DNA panel for genomic studies in Russian cattle breeds. Russian Journal of Genetics: Applied Research. 5(1):21-25.

Interpretive Summary: Identifying novel SNPs in the genomes of Russian cattle is expected to improve the efficiency of genomic selection and pinpoint mutations that directly affect quantitative traits in this population. Toward that aim, a diverse panel of 96 DNA samples was assembled from beef and dairy cattle across Russia. Results showed this panel was comparable to that developed by the USDA for use in beef cattle. This Russian Cattle Genomic Diversity Panel (RCGDP version 1.0) is available and can be used to develop simple genetic tests for large-scale SNP genotyping and analyses of genetic polymorphisms.

Technical Abstract: A panel of 96 DNA samples (Russian Cattle Genomic Diversity Panel 1.0 or RCGDP 1.0) characterizing the breadth of genetic diversity in popular Russian cattle breeds was designed. The panel contains from four to eight animals from each of 11 dairy and six dairy-meat and meat breeds. The main criterion for inclusion of individual animals in the panel was that they had, according to the pedigree data, the greatest number of unrelated haploid genomes. The resulting panel of 96 animals was estimated to contain 186.1 unshared haploid genomes and was expected to allow a 95% probability of detecting alleles with a frequency greater than 0.016 in the group. This is comparable to the USDA MARC Beef Cattle Diversity Panel version 2.1. Analysis of three SNPs in the Galloway, Hereford, Grey Ukrainian and Black Pied breeds showed no significant differences in the allele frequencies between the animals in the panel and in the herds. Thus, the designed DNA panel may be useful for SNP selection in the genetic analysis of economically important traits, for identification of purebred and hybrid animals, and, probably, for tentative estimation of allele frequencies in the populations.