Skip to main content
ARS Home » Southeast Area » Tifton, Georgia » Crop Protection and Management Research » Research » Publications at this Location » Publication #311820
Title: QTL mapping & quantitative disease resistance to TSWV and leaf spots in a recombinant inbred line population SunOleic 97R and C94022 of peanut (Arachis hypogaea L.)

Author
item KHERA, PAWAN - University Of Georgia
item WANG, HUI - University Of Georgia
item CULBREATH, ALBERT - University Of Georgia
item PANDEY, MANISH - International Crops Research Institute For Semi-Arid Tropics (ICRISAT) - India
item VARSHNEY, RAJEEV - International Crops Research Institute For Semi-Arid Tropics (ICRISAT) - India
item WANG, XINGJUN - Genome Sequencing Center
item LIAO, BOSHOU - Genome Sequencing Center
item ZHANG, XINYOU - Genome Sequencing Center
item WANG, JIANPING - University Of Florida
item Holbrook, Carl - Corley
item Guo, Baozhu

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 11/8/2014
Publication Date: 11/10/2014
Citation: Khera, P., Wang, H., Culbreath, A.K., Pandey, M.K., Varshney, R., Wang, X., Liao, B., Zhang, X., Wang, J., Holbrook Jr, C.C., Guo, B. 2014. QTL mapping & quantitative disease resistance to TSWV and leaf spots in a recombinant inbred line population SunOleic 97R and C94022 of peanut (Arachis hypogaea L.). Meeting Abstract. Advances in Arachis through Genomics and Biotechnology (AAGB) meeting, November 10-14, 2014, Savannah, Georgia.

Interpretive Summary:

Technical Abstract: Peanut is susceptible to a range of diseases, such as Tomato spotted wilt virus (TSWV), early leaf spot (ELS) and late leaf spot (LLS). Breeding line NC94022 has been identified with the highest resistance to TSWV in the field. Quantitative trait loci (QTL) mapping is a highly effective approach for studying genetically controlled disease resistance. A population of 352 recombinant inbred lines (RILs) from a cross between ‘SunOleic 97R’ and ‘NC94022’ was developed. A genetic map was constructed with 248 SSR marker loci across 21 linkage groups (LGs) and a total map length of 1425.91cM with a marker interval of 5.7 cM. QTL analysis using phenotypic data from multiple-locations and years (2009-2013) resulted in the identification of 89 QTLs on 11 LGs with phenotypic variance (PV) ranging from 3.46% to 30.15%, and 22 QTLs were found to be major QTLs (PV >10%), including 41 (10 major ones on 9 LGs), 35 (eight major ones on 6 LGs) and 13 (four major ones on 3 LGs) QTLs for resistances to ELS, LLS, and TSWV, respectively. Consistent QTL regions expressed in more than two environments were also identified for all three diseases on LG1 (PV up to 15%), LG13 (PV up to 17%) and LG1 (PV up to 29%), respectively. It was interesting to note a “gene rich” LG for ELS and TSWV in LG1. Further fine-mapping of the QTLs will be needed in order to identify the potential resistance gene(s) and decipher the molecular basis of disease resistance mechanisms in peanut.