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ARS Home » Southeast Area » Tifton, Georgia » Crop Protection and Management Research » Research » Publications at this Location » Publication #311800
Title: Combining genomic approaches to understand genetic control of aflatoxin contamination in peanut

Author
item NAYAK, SPURTHI - International Crops Research Institute For Semi-Arid Tropics (ICRISAT) - India
item SUDINI, HARI - International Crops Research Institute For Semi-Arid Tropics (ICRISAT) - India
item AGARWAL, GAURAV - International Crops Research Institute For Semi-Arid Tropics (ICRISAT) - India
item PANDEY, MANISH - International Crops Research Institute For Semi-Arid Tropics (ICRISAT) - India
item UPADHYAYA, HARI - International Crops Research Institute For Semi-Arid Tropics (ICRISAT) - India
item JANILA, PASUPULETI - International Crops Research Institute For Semi-Arid Tropics (ICRISAT) - India
item HAMIDOU, FALALOU - International Crops Research Institute For Semi-Arid Tropics (ICRISAT) - Nigeria
item DESMAE, HAILE - International Crops Research Institute For The Semi-Arid Tropics (ICRISAT)
item OZIAS-AKINS, PEGGY - University Of Georgia
item Guo, Baozhu
item VARSHNEY, RAJEEV - International Crops Research Institute For Semi-Arid Tropics (ICRISAT) - India

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 11/8/2014
Publication Date: 11/10/2014
Citation: Nayak, S.N., Sudini, H.K., Agarwal, G., Pandey, M.K., Upadhyaya, H.D., Janila, P., Hamidou, F., Desmae, H., Ozias-Akins, P., Guo, B., Varshney, R.K. 2014. Combining genomic approaches to understand genetic control of aflatoxin contamination in peanut. Meeting Abstract. Advances in Arachis through Genomics and Biotechnology (AAGB), November 10-14, 2014, Savannah, Georgia.

Interpretive Summary:

Technical Abstract: Aflatoxin contamination in peanut is more prevalent under rainfed conditions making produce unfit for human and animal consumption, affecting the international trade adversely. Although the losses in yield and quality due to aflatoxin contamination is higher than realized, there are limited resistance resources available to use in aflatoxin resistance breeding. Hence, attempts are being made to use different genomic approaches to tackle this challenge. In the first approach, transcriptome sequencing of resistant (J 11, ICGV 91278) and susceptible (JL 24) genotypes has been carried out to study the resistance to in-vitro and pre-harvest colonization of seeds by Aspergillus flavus (strain AF-11F). The sequencing of transcripts generated 64 to 94 million reads using Illumina HiSeq 2000 platform in case of in-vitro seed colonization. The differentially expressed reads will be analyzed to identify the candidate resistant genes and understanding the underlying mechanisms. In the second approach, peanut ‘reference set’ was screened for aflatoxin contamination across four environments at Niger and the analysis is underway to identify marker-trait associations for aflatoxin contamination. As there are no bi-parental populations available for aflatoxin resistance in peanut, multi-parent advanced generation intercross (MAGIC) populations are being developed for aflatoxin resistance along with other important agronomic traits as the third approach. The comprehensive efforts to identify marker-trait associations and the transcripts responsible for varying resistance to aflatoxin will be a useful resource to address this serious constraint.