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ARS Home » Midwest Area » Madison, Wisconsin » Vegetable Crops Research » Research » Publications at this Location » Publication #308416
Title: Developing resources for diploid potato breeding and genetics

Author
item Jansky, Shelley
item ENDELMAN, JEFF - University Of Wisconsin
item COOMBS, JOSEPH - Michigan State University
item MANRIQUE, NORMA - Michigan State University
item DOUCHES, DAVID - Michigan State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 8/22/2014
Publication Date: 11/4/2014
Citation: Jansky, S.H., Endelman, J., Coombs, J., Manrique, N., Douches, D. 2014. Developing resources for diploid potato breeding and genetics. SOL 2014. Paper No. VI-3.

Interpretive Summary:

Technical Abstract: The cultivated potato (Solanum tuberosum Gp. tuberosum) is an asexually propagated cross-pollinated autotetraploid crop, for which breeding methodology has not changed in 100 years. Current methods for breeding potato cultivars are genetically inefficient due to polyploidy, resource intensive due to vegetable propagation, and do not take maximum advantage of rapidly expanding genomics resources. Most diploid potatoes are self-incompatible, but the dominant Sli gene allows self-pollination. By exploiting self-compatibility from diploid clones carrying Sli, we are creating diploid inbred lines for potato breeding and genetics research. Our current research is developing a series of recombinant inbred line populations that can be used for future mapping studies and the production of inbred lines for breeding diploid cultivars. In the first true F2 population (DM1-3 x M6) the dominant self-compatibility trait from S. chacoense is being mapped. From a total of 211 progeny 106 progeny were self-compatible, 31 were self-incompatible, 44 were male sterile and 30 are currently undefined. SNP genotyping has been completed on 94 progeny using the Infinium 8303 potato SNP array. There were 1633 segregating SNPs in F2 population of which 800 segregated independently. The self-compatibility trait mapped to the long arm of chromosome II. The F2 generation of another RIL population produces tuber yields equivalent to major potato cultivars. In other work, a sample of 50 diploid lines and dihaploids of cultivated potatoes have been SNP genotyped to understand relationships and the genetic diversity of the diploid germplasm in comparison to the North American cultivated tetraploid germplasm. We believe it is possible to convert potato into a diploid crop based on inbred lines and propagated by true seed.