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ARS Home » Northeast Area » Boston, Massachusetts » Jean Mayer Human Nutrition Research Center On Aging » Research » Publications at this Location » Publication #304354
Title: Comparison of tissue concentrations in male and female C57BL/6 mice

Author
item HARSHMAN, STEPHANIE - Jean Mayer Human Nutrition Research Center On Aging At Tufts University
item SMITH, DONALD - Jean Mayer Human Nutrition Research Center On Aging At Tufts University
item KARL, PHILIP - Jean Mayer Human Nutrition Research Center On Aging At Tufts University
item SHEN, XIAOHUA - Jean Mayer Human Nutrition Research Center On Aging At Tufts University
item BOOTH, SARAH - Jean Mayer Human Nutrition Research Center On Aging At Tufts University
item FU, XUEYAN - Jean Mayer Human Nutrition Research Center On Aging At Tufts University

Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: 2/13/2014
Publication Date: 4/1/2014
Citation: Harshman, S., Smith, D., Karl, P.J., Shen, X., Booth, S., Fu, X. 2014. Comparison of tissue concentrations in male and female C57BL/6 mice [abstract]. Journal of Federation of American Societies for Experimental Biology. 28:1041.4.

Interpretive Summary:

Technical Abstract: The tissue-specific response to dietary vitamin K (VK) manipulation has not been well studied in mice. This limits the use of genetically modified mouse models in VK studies. The objective of this study was to determine the sex-specific effects of dietary VK manipulation on serum, liver and extra-hepatic tissue VK concentrations in C57BL/6 mice (n=64). Mice were weight-matched and pair-fed a control diet (1.4+/-0.08 mg phylloquinone (PK)/kg) or VK-deficient diet (31±0.45 micrograms PK/kg) for 28 days. Liver, kidney, brain, pancreas, adipose tissue, and serum PK and menaquinone-4 (MK-4) concentrations were measured by HPLC. Data were log transformed and analyzed by a general linear model, stratified by diet. Male and female mice responded differently to dietary manipulation of VK in a tissue-dependent manner (for all sex by diet interactions p less than or equal to 0.031). Serum PK concentrations did not differ between males and females within each diet (p greater than or equal to 0.95); no serum MK-4 was detected. Females on the control diet had significantly higher PK and MK-4 concentrations in adipose tissue, kidney, liver, pancreas, and brain compared to males (p less than or equal to 0.01). Only MK-4 concentrations in adipose tissue, liver, and pancreas did not differ between males and females on the deficient diet (p greater than or equal to 0.44). Sex-specific differences in response to VK manipulation need to be considered when using animal models of VK metabolism.