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ARS Home » Southeast Area » Stuttgart, Arkansas » Dale Bumpers National Rice Research Center » Research » Publications at this Location » Publication #303634

Title: Development of practical diagnostic methods for monitoring rice bacterial panicle blight disease and evaluation of rice germplasm for resistance

Author
item WAMISHE, YESHI - University Of Arkansas
item Jia, Yulin
item KELSEY, S - University Of Arkansas
item BELMAR, SCOTT - University Of Arkansas
item RASHEED, M - University Of Arkansas

Submitted to: Arkansas Agricultural Experiment Station Research Series
Publication Type: Experiment Station
Publication Acceptance Date: 5/20/2014
Publication Date: 8/3/2014
Citation: Wamishe, Y., Jia, Y., Kelsey, S., Belmar, S., Rasheed, M.D. 2014. Development of practical diagnostic methods for monitoring rice bacterial panicle blight disease and evaluation of rice germplasm for resistance. Arkansas Agricultural Experiment Station, B.R. Wells Rice Research Studies-20012, eds. Norman, R.J., and Moldenhauer, K., series 609:103-108. (http://arkansasagnews.uark.edu/609-13.pdf).

Interpretive Summary:

Technical Abstract: A study was initiated to understand Burkholderia glumae (major causal agent for bacterial panicle blight disease of rice) to develop practical diagnostic methods for monitoring the disease; and to evaluate rice germplasm for resistance. B. glumae was frequently isolated from symptomatic panicles on CCNT, a semi-selective medium. Selected isolates were assessed for virulence using hypersensitivity reaction on wild tobacco leaves and pathogenicity tests on rice seedlings. B. glumae isolates found to be hypersensitive and pathogenic were used to inoculate seedlings in the greenhouse for bioassay studies and to screen germplasm for resistance in the field. The isolates were stored at -80°C in 25% glycerol. In 2013, two hundred of URRN and ninety of ARPT entries were inoculated between boot-split to flowering growth stage of rice. Inoculation was done twice in an interval of 4 to 5 days. A 0 to 9 disease scoring scale was used where 0 showed no disease and 9 to severe bacterial panicle blight. Of 290 entries, 15 entries showed no symptom of the disease and 53 entries showed moderate resistance with a rating of 1 to 5. The remaining entries rated between 6 and 9 and were grouped as moderately susceptible to very susceptible. The cutoff point between moderately resistant and moderately susceptible was based on the reaction of the known moderately resistant Jupiter variety that rated 5 for disease. None of the greenhouse seedling inoculations were definitive enough to separate relative resistance levels among the varieties tested. Detached leaf inoculation are being modified and tested in search of a technique that provides consistent resistance or susceptibility reaction among varieties. Additional tests, including for molecular marker search, will continue for one more year.