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ARS Home » Southeast Area » Stuttgart, Arkansas » Dale Bumpers National Rice Research Center » Research » Publications at this Location » Publication #300955
Title: Elucidation of molecular dynamics of invasive species of rice

Author
item LIU, YAN - University Of Arkansas
item QIN, XINSHUAI - Washington University
item VIGUEIRA, CINDY - University Of Massachusetts
item Jia, Melissa
item Jia, Yulin
item Gealy, David
item OLSEN, KEN - Washington University
item CAICEDO, ANA - University Of Massachusetts
item BURGOS, NILDA - University Of Arkansas

Submitted to: Rice Technical Working Group Meeting Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 2/18/2014
Publication Date: 12/19/2014
Citation: Yan, L., Qin, X., Vigueira, C., Jia, M.H., Jia, Y., Gealy, D.R., Olsen, K., Caicedo, A., Burgos, N. 2014. Elucidation of molecular dynamics of invasive species of rice. Proc. 35th Rice Tech. Work. Group Meet., New Orleans, LA, p.94. Feb 18-21, 2014. CDROM.

Interpretive Summary:

Technical Abstract: Cultivated rice fields are aggressively invaded by weedy rice in the U.S. and worldwide. Weedy rice results in loss of yield and seed contamination. The molecular dynamics of the evolutionary adaptive traits of weedy rice are not fully understood. To understand the molecular basis and identify the important genes /quantitative trait loci (QTLs) which are associated with weedy traits, two recombinant inbred line (RIL) populations were made between two weedy rice ecotypes, a straw hull weedy rice type 1135-01 (RR9) and a black hull type 1996-9 (RR20), and a putative evolutionarily close relative, the Asian indica rice variety Dee Geo Woo Gen (DGWG). The populations derived from the cross of RR9 and DGWG and the cross of RR20 and DGWG consist of 185 and 234 individuals, respectively. Both F5 RIL populations were genotyped using next generation Genotype by Sequencing (GBS) method. The phenotype of nine traits including emergence date, heading date, chlorophyll content, seed shattering, plant height, plant type, panicle type, panicle length, and awns of two F6 and F7 RILs populations were evaluated in replicated field plot experiments in the summers of 2012 and 2013 . Seeds traits of one RIL population from the cross of RR20 and DGWG including bran color, grain length, grain width, and kernel weight are being evaluted in the lab using 2312 Graincheck. Disease reactions of all individuals of both RIL populations to the common races of U.S. rice blast, Magnaporthe oryzae, field isolates, IB54, IE1(ZN13), IE1K (TM2), ID1 (ZN42), and IB33, were determined in a greenhouse. Seedling height and culm color of all the RILs from the two populations were also evaluated in the greenhouse. The genomic regions associated with agronomic traits and with resistance to rice blast disease are being established by associating phenotypic and genotypic data generated using GBS. The haplotypes harboring important candidate gene(s) within QTLs are being analyzed and the co-segregation of molecular markers are being identified for important traits. The genetic basis of beneficial traits for crop improvement, insights into the molecular dynamics of the evolution of invasive weedy biotypes, and the implications for weed management will be presented.