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ARS Home » Pacific West Area » Davis, California » Nat'l Clonal Germplasm Rep - Tree Fruit & Nut Crops & Grapes » Research » Publications at this Location » Publication #299730
Title: Cloning: plants – micropropagation/tissue culture

Author
item REED`, PAUL - University Of Nebraska
item Preece, John

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 1/23/2013
Publication Date: N/A
Citation: N/A

Interpretive Summary: Clonal micropropagation is the multiplication of the buds and shoots that occur in leaf axils on a defined nutrient medium in an aseptic in vitro environment. The resulting shoots are either subdivided for continued multiplication or rooted and acclimatized to the greenhouse or field. Micropropagation is a continuous process that is commonly divided into 5 stages: Stage 0, Stock plants; Stage I, Establishment; Stage II, Multiplication; Stage III, Pretransplant stage, rooting; and Stage IV, Acclimatization. Each stage depends on the previous one, but has very different objectives and requirements all leading to the production of genotypically and phenotypically uniform plants.

Technical Abstract: Clonal micropropagation is the multiplication of the buds and shoots that occur in leaf axils on a defined nutrient medium in an aseptic in vitro environment. The resulting shoots are either subdivided for continued multiplication or rooted and acclimatized to the greenhouse or field. Micropropagation is a continuous process that is commonly divided into 5 stages: Stage 0, Stock plants; Stage I, Establishment; Stage II, Multiplication; Stage III, Pretransplant stage, rooting; and Stage IV, Acclimatization. Each stage depends on the previous one, but has very different objectives and requirements all leading to the production of genotypically and phenotypically uniform plants.