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ARS Home » Southeast Area » Florence, South Carolina » Coastal Plain Soil, Water and Plant Conservation Research » Research » Publications at this Location » Publication #296557
Title: Submission to NCBI Sequence Read Archive (SRA): Raw read files from manuscript “RNA-seq transcriptome profiling of upland cotton (Gossypium hirsutum) root tissue under water deficit stress” NCBI SRA Accession No. PRJNA210770

Author
item Bowman, Megan
item Park, Wonkeun
item Bauer, Philip
item UDALL, J. - Brigham Young University
item PAGE, J. - Brigham Young University
item RANEY, J. - Brigham Young University
item Scheffler, Brian
item Campbell, Benjamin - Todd

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Other
Publication Acceptance Date: 8/2/2013
Publication Date: 12/6/2013
Citation: Bowman, M.J., Park, W., Scheffler, B.E., Bauer, P.J., Campbell, B.T., Page, J.T., Udall, J.A., Raney, J. 2013. Submission to NCBI Sequence Read Archive (SRA): Raw read files from manuscript “RNA-seq transcriptome profiling of upland cotton (Gossypium hirsutum) root tissue under water deficit stress” NCBI SRA Accession No. PRJNA210770. National Center for Biotechnology Information (NCBI). National Center for Biotechnology Information. SRA PRJNA210770.

Interpretive Summary: This report describes the isolation, RNA sequencing and submission of 50 base pair(bp) Illumina RNA seq raw sequence reads with differential expression patterns in response to water deficit stress. The genes provide genetic targets to manipulate the water use characteristics of cotton. Raw sequence reads were deposited into a publicly available database at the National Center for Biotechnology Information Sequence Read Archive (NCBI SRA).

Technical Abstract: Gossypium hirsutum L. cultivar Siokra L-23 was grown under rainfed and irrigated treatments in a field with very sandy soil. Root samples were collected for each treatment during the reproductive growth period. The RNA of all root tissues was used in an RNA-seq transcriptome profiling study with an objective to evaluate global gene expression changes occurring in root tissue under water deficit stress. We report the submission of raw 50 bp Illumina HiSeq next generation sequencing reads to the National Center for Biotechnology Information Sequence Read Archive (NCBI SRA). These data have provided potential genetic targets to manipulate the water use characteristics of cotton at the molecular level. Interpretive abstract: This report describes the isolation, RNA sequencing and submission of 50 bp Illumina RNA seq raw sequence reads with differential expression patterns in response to water deficit stress. The genes provide genetic targets to manipulate the water use characteristics of cotton. Raw sequence reads were deposited into a publicly available database at the National Center for Biotechnology Information Sequence Read Archive (NCBI SRA).