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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Functional Foods Research » Research » Publications at this Location » Publication #293120
Title: Yerba mate tea and mate saponins prevented azoxymethane-induced inflammation of rat colon through suppression of NF-kB p65ser(311) signaling via IkB-a and GSK-3ß reduced phosphorylation

Author
item PUANGPRAPHANT, SIRIMA - Chulalongkorn University
item DIA, VERMONT - University Of Illinois
item GONZALEZ DE MEJIA, ELVIRA - University Of Illinois
item GARCIA, GUADALUPE - University Of Illinois
item Berhow, Mark
item WALLIG, MATTHEW - University Of Illinois

Submitted to: Biofactors
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/10/2012
Publication Date: 3/29/2013
Citation: Puangpraphant, S., Dia, V.P., Gonzalez de Mejia, E., Garcia, G., Berhow, M.A., Wallig, M.A. 2013. Yerba mate tea and mate saponins prevented azoxymethane-induced inflammation of rat colon through suppression of NF-kB p65ser(311) signaling via IkB-a and GSK-3ß reduced phosphorylation. BioFactors. 39(4):430-440.

Interpretive Summary: Yerba mate tea (YMT) has a chemical prevention role in a variety of inflammatory diseases that lead to cancer. The objective was to determine the capability of YMT and mate saponins to prevent a chemically induced colonic inflammation in rats. YMT (2% dry leaves, w/v, as a source of drinking fluid) and mate saponins (0.01% in the diet, at a concentration presnt in one cup of YMT) were given to rats 2 weeks prior to cheimcal cancer induction until the end of the study; while control rats received a basal diet and drinking water. After 8-weeks of study, the colons were examined and were cut into three equal sections and were analyzed for cancer markers. YMT reduced cancer cell formation from 113 (control group) to 89 (P < 0.05). YMT and mate saponins reduced the expression of proinflammatory molecules with concomitant reduction in the enzyme marker p-p65 (P < 0.05). Immunohistochemical analysis of the middle colons showed that YMT and mate saponins reduced the expression of one marker enzyme by 45.7% and 43.1%, respectively, in comparison to the control (P < 0.05). In addition, the expression of molecules upstream was downregulated by YMT 24.7% and 24.4%, respectively (P < 0.05). Results suggest the mechanism involved in the chemopreventive effect of YMT and mate saponin consumption in AOM induced-colonic inflammation in rats is through inhibition of NF-kB.

Technical Abstract: Yerba mate tea (YMT) has a chemopreventive role in a variety of inflammatory diseases. The objective was to determine the capability of YMT and mate saponins to prevent azoxymethane (AOM)-induced colonic inflammation in rats. YMT (2% dry leaves, w/v, as a source of drinking fluid) (n = 15) and mate saponins (0.01% in the diet, at a concentration present in one cup of YMT) (n = 15) were given ad libitum to rats 2 weeks prior to AOM-injection until the end of the study; while control rats (n = 15) received a basal diet and drinking water. After 8-weeks of study, total colonic mucosa was scraped (n 5 3 rats/group) and the remaining colons (n = 12 rats/group) were cut into three equal sections and aberrant crypt foci (ACF) were analyzed. YMT reduced ACF formation from 113 (control group) to 89 (P < 0.05). YMT and mate saponins reduced the expression of proinflammatory molecules COX-2 and iNOS with concomitant reduction in p-p65 (P < 0.05). Immunohistochemical analysis of the formalin-fixed middle colons showed that YMT and mate saponins reduced the expression of p-p65^ser311^ by 45.7% and 43.1%, respectively, in comparison to the control (P < 0.05). In addition, the expression of molecules upstream of NF-kB such as p-IkB-a and p-GSK-3ß^Y216^ was downregulated by YMT 24.7% and 24.4%, respectively (P < 0.05). Results suggest the mechanism involved in the chemopreventive effect of YMT and mate saponin consumption in AOM induced-colonic inflammation in rats is through inhibition of NF-kB.